Extension of the NoKo Multi-Class Veterinary Drug Residue Analysis Method by Liquid Chromatography Mass Spectrometry for Egg and Milk

Hütteroth A., Bresching A., Seidl G., Vögeding K., Winkler T., Witt G.
Extension of the NoKo Multi-Class Veterinary Drug Residue Analysis Method by Liquid Chromatography Mass Spectrometry for Egg and Milk
Poster im Rahmen der Konferenz EURORESIDUES IX 2022, 23. – 25.05.2022

The Multi-class veterinary drug residue analysis method developed in 2017 by the North German Cooperation of State Laboratories (NOKO) [1] is extended for the matrices egg and milk. The goal is an enlarged method for the analysis of antibiotics and other veterinary drugs of relevance in all matrices of the national control plan. With the matrices egg and milk, the next step is been taken to achieve this goal.
The poster will illustrate the studies conducted for sample pretreatment and cleanup for egg and milk, the concept of screening validation and the validation results for all analyts. The classes included are amphenicols, ansamycines, cephalosporins, quinolones, diaminopyrimidines, glucocorticoides, lincosamides, macrolides, nitroimidazoles, nonsteroidal anti-inflammatory drugs, penicillins, pleuromutilins, polypeptides, sulfonamides, tetracyclines and virginiamycin.
The validation results show the suitability of the method to screen 108 and 119 different veterinary drugs in milk and egg respectively. The method is robust for routine analysis according to Commission Implementation Regulation (EU) 2021/808 [2].

Literatur
[1] Hütteroth A, Christof O, Schoknecht B: Tierarzneimittelrückstände – Multimethode; Entwicklung einer Screeningmethode im Rahmen der Norddeutschen Kooperation. Deut Lebensm-Rundsch 115, 403-408 (2019).
[2] COMMISSION IMPLEMENTING REGULATION (EU) 2021/808 of 22 March 2021 on the performance of analytical methods for residues of pharmacologically active substances used in food-producing animals and on the interpretation of results as well as on the methods to be used for sampling and repealing Decisions 2002/657/EC and 98/179/EC.